二抗在免疫学实验中起着举足轻重的作用,二抗的选择也决定了整个实验的成败。实验中在选择二抗时必须注意以下几点:(1) 一抗种属来源,主要根据一抗种属来源来决定确定二抗的类型,如一抗是小鼠来源,那二抗就买抗小鼠的即可(如羊抗小鼠、兔抗小鼠等均可)。
(2)标记物的选择。一般来讲,耦联到二抗上的探针主要有酶(辣根过氧化酶HRP和碱性磷酸酶AP或其衍生物APAAP,PAP),荧光基团(FITC, RRX, TR, PE)和生物素。选用哪种探针的二抗主要取决于具体的实验。对于western blot和ELISA,常用的二抗是酶标二抗,而细胞或组织标记实验(细胞免疫化学,组织免疫化学,流式细胞术)中通常使用荧光基团标记的二抗,免疫组化中也可以使用辣根过氧化酶或碱性磷酸酶标记的二抗。如果想要更大程度的放大检测信号,可以使用biotin/avidin检测系统。
友科生物可以提供多种常规二抗,质优价廉,欢迎选购。
编号 | 识别的一抗种属 | 名称 | 应用 | 规格 |
YKGP201H | MOUSE IgG,HRP | Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP201F | MOUSE IgG,FITC | Anti-MOUSE IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP201B | MOUSE IgG,BIOTIN | Anti-MOUSE IgG (H&L) (GOAT) Antibody Biotin Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP202H | RABBIT IgG,HRP | Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP202F | RABBIT IgG,FITC | Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP202B | RABBIT IgG,BIOTIN | Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP203H | HUMAN IgG,HRP | Anti-HUMAN IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP203F | HUMAN IgG,FITC | Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP203B | HUMAN IgG,BIOTIN | Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKRP204H | GOAT IgG,HRP | Anti-GOAT IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKRP204F | GOAT IgG,FITC | Anti-GOAT IgG (H&L) (RABBIT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKRP204B | GOAT IgG,BIOTIN | Anti-GOAT IgG (H&L) (RABBIT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml |
电话:021-58955621
咨询:youke_sh@sina.com
地址:蔡伦路720号1号楼339室